Yeah. My Makerfarm Prusa i3 (without the v) has done it to me. I'm slicing with Slic3r and running through Pronterface. I seem to remember once this happened so I printed the same Gcode from an SD card without problems.

I have a bad time with my Pronterface not recognising the printer via the COMM port. I think the problem resides somewhere in drivers since the Pronterface drivers are unsigned and the Windows O/S doesn't like unsigned drivers. Probably part of the built in anti-virus protection in the later versions of Windows.

I know that when I was installing Pronterface, I had to deal with the Python files a lot.

Old Man Emu