My guess would be that your Y bed is warping during your print and the bed rollers are hitting the support on the back of the printer.

Quote Originally Posted by BLKKROW View Post
Recently I decided to print 3 objects at once instead of 1 at a time. This resulted in the print time reaching 10 hours, which is the longest I have ever printed on the Prusa.

However, as you can see in the attachment on a layer the print was shifted and completed the print shifted. I have checked Simplify 3D and there is no layer shift and I have printed at this height many times, I have printed this part probably 10-15 times, but just one at a time.

Can anyone give me an idea of what may have caused this? Could it be the stepper drivers over heating? That is my only thought.