You have a Prusa i3 I would think that you would want to use the best slicer PrusaSlicer 2.3.0 rc2 Has configurations for your 3D Printer.
Without the correct profiles any thing could happen.

Upon down loading PrusaSlicer and installing.
1st Run Select Configuration > Configuration Wizard > Prusa FFF and select your printer profile after you can select from many filaments.