It is possible that the Y axis could be losing steps somehow?

1. What speed are you printing at? Is it too fast? Try dialing your speed back to something like 30mm/s.
2. Are the steppers getting enough power? This machine looks like a Robo3d R1 plus. Is this built or bought pre-assembled? In any case, can you adjust the current to your stepper drivers somehow?
3. Do you ever hear/see any belts skipping? They would likely make some fair noticable noises if they were slipping/skipping.
4. Does your bed move freely when the machine is powered off and you move it by hand? Are there any areas along the bed axis where it feels tighter (takes more force) to move the bed?

For more in-depth info Take a look here:
https://www.simplify3d.com/support/p...r-misalignment